Titlebook: Complement Methods and Protocols; B. Paul Morgan Book 2000 Humana Press 2000

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书目名称Complement Methods and Protocols影响因子(影响力)




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书目名称Complement Methods and Protocols网络公开度学科排名




书目名称Complement Methods and Protocols被引频次




书目名称Complement Methods and Protocols被引频次学科排名




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书目名称Complement Methods and Protocols年度引用学科排名




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Purification of Complement Components, Regulators, and Receptors by Classical Methods,ilable in most biochemistry laboratories. None of these methods require the large amounts of specific antibodies needed for the popular and rapid immunoaffinity methods to be described in .. A further advantage of classical chromatography is that the methods described in this chapter for human C can

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Immunoaffinity Methods for Purification of Complement Components and Regulators,rs, and regulators have been successfully isolated by immunoaffinity protocols. The use of these methods is limited only by the availability of suitable antibodies in sufficient quantities (.–.). Immunoaffinity methods are compatible with most detergents used in the solubilization of membrane protei

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Immunochemical Measurement of Complement Components and Activation Products,ent proteolytic cleavage products, recruited from these inactive precursor molecules in a sequentially proceeding cascade (.). This leads to an alteration of their antigenic pattern and thus to the consumption of the nativemolecule. Concomitantly, complement activation initiates formation of multi-m

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Complement Deposition in Tissues,and are detected by immunohistochemical methods. C3b, C4b, and their fragments remain covalently bound on targets and components of the membrane attack complex (MAC) can become inserted into cell membranes. In addition, many components (like C1q and properdin) are stable enough and have such a high

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Complement Regulators and Receptors in Tissues,th appropriate antibodies. The bound antibodies are visualized with a proper label that has been attached to the primary antibody itself or to a secondary antibody against the primary immunoglobulin (..). Despite the label the antibody retains its reactivity with its antigen. In immunofluorescence m

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Measurement of C3 Fragment Deposition on Cells, or regulation. However, other consequences of complement activation are probably of more relevance in vivo. Complement plays a major role in enhancing the generation of specific antibodies and provides an interface between the humoral and cellular immune responses (.–.). Complement also increases t

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