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Titlebook: Co-Immunoprecipitation Methods for Brain Tissue; Yuji Odagaki,Dasiel O. Borroto-Escuela Book 2019 Springer Science+Business Media, LLC, pa

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发表于 2025-3-21 19:59:59 | 显示全部楼层 |阅读模式
书目名称Co-Immunoprecipitation Methods for Brain Tissue
编辑Yuji Odagaki,Dasiel O. Borroto-Escuela
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Neuromethods
图书封面Titlebook: Co-Immunoprecipitation Methods for Brain Tissue;  Yuji Odagaki,Dasiel O. Borroto-Escuela Book 2019 Springer Science+Business Media, LLC, pa
描述.This volume covers various aspects of co-immunoprecipitation (co-IP) methods and its relevant use in studying protein-protein interactions (PPIs) in health and diseases of the Central Nervous System. The chapters in this book discuss topics such as using co-IP to detect G protein-coupled receptors (GPCR), receptor tyrosine kinases (RTK) and ion channels heteroreceptor complexes in brain tissue; the histoblot technique; interaction strength between synaptic proteins using COS-7; and co-IP analysis of the protein-protein interactions in the neurons of Polymita. In .Neuromethods. series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory..Cutting-edge and thorough, .Co-Immunoprecipitation Methods for Brain Tissue. is a valuable resource for any researcher interesting in learning more about this developing field..
出版日期Book 2019
关键词PPI assay; multiprotein complexes; protein-protein interactions; antibody; lysate
版次1
doihttps://doi.org/10.1007/978-1-4939-8985-0
isbn_ebook978-1-4939-8985-0Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
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,Co-immunoprecipitation Methods to Identify Associated Proteins with Estrogen Receptor α at Postsynaficity and decreasing false-positive result. In the current work, we show a robust procedure where the use of two sequential approaches (separation of postsynaptic density by centrifuge separation method in combination with detergent treatment and the use of specific antibodies against target protei
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Using Co-immunoprecipitation and Shotgun Mass Spectrometry for Protein-Protein Interaction Identifi related to the target such as its function, regulation, and mechanisms of action. Co-immunoprecipitation (co-IP) followed by shotgun proteomics is a powerful technique for capturing and identifying endogenous protein complexes. Although it is a simple concept, performing the experiment can be chall
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Co-immunoprecipitation Analysis of GPCR Complexes in the Central Nervous System,re suggested to occur in many neural cells, their contribution to neuronal functions remains unclear. We address this question using a combination of physiological techniques and co-immunoprecipitation (Co-IP), which is one of the most commonly used methods for determining whether a target protein c
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