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Titlebook: Clinical Proteomics; Methods and Protocol Antonia Vlahou Book 2008 Humana Press 2008 2D electrophoresis.Clinical proteomics.Computational b

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发表于 2025-3-21 17:43:29 | 显示全部楼层 |阅读模式
书目名称Clinical Proteomics
副标题Methods and Protocol
编辑Antonia Vlahou
视频video
概述Compiled by experts in the field, these protocols demonstrate the widespread use of proteomic techniques used in clinical bench work..Step-by-step instructions that ensure successful results.Integrate
丛书名称Methods in Molecular Biology
图书封面Titlebook: Clinical Proteomics; Methods and Protocol Antonia Vlahou Book 2008 Humana Press 2008 2D electrophoresis.Clinical proteomics.Computational b
描述.The field of clinical proteomics has rapidly evolved during the past few years and is continuously growing as new methodologies and technologies emerge. In .Clinical Proteomics., a select group of leading researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. Experimental approaches involving the application of two-dimensional electrophoresis, multi-dimensional liquid chromatography, SELDI/MALDI mass spectrometry and protein arrays, as well as the bioinformatic and statistical tools pertinent to the analysis of proteomics data, are thoroughly detailed in these readily reproducible methods and protocols, explained through the step-by-step instructional format of the highly successful .Methods in Molecular Biology™ .series...With its multi-disciplinary approach, .Clinical Proteomics .provides integrated, cutting-edge techniques sure to be invaluable to a wide range of researchers including clinicians, molecular biologists, chemists, bioinformaticians and computational biologists..
出版日期Book 2008
关键词2D electrophoresis; Clinical proteomics; Computational biology; Polyacrylamid-Gelelektrophorese; Proteom
版次1
doihttps://doi.org/10.1007/978-1-59745-117-8
isbn_softcover978-1-61737-799-0
isbn_ebook978-1-59745-117-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2008
The information of publication is updating

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Specimen Collection and Handlingrate the desired specimen. The choice of blood specimens, its collection, handling, processing, and storage are important aspects since these characteristics can have a tremendous impact on the results of the analysis..The awareness of clinical practices in medical laboratories and the current knowl
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Tissue Sample Collection for Proteomics Analysisllection for proteomic purposes with emphasis on the most important steps, including timing issues and the procedures for immediate freezing, storage, and microdissection of the cells of interest or “tissue targets” and the lysates for protein isolation for SELDI, MALDI, and 2DGE applications. The p
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Protein Profiling of Human Plasma Samples by Two-Dimensional Electrophoresisr their corresponding enzyme activities may reflect either a healthy or a diseased state. Given that there is no defined genomic information as to the intact protein components in plasma, protein profiling could be the first step toward its molecular characterization. Several problems exist in the a
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MALDI/SELDI Protein Profiling of Serum for the Identification of Cancer Biomarkers combine higher throughput with the ability to observe differential protein expression levels, have been applied to this goal. An example of such a system is the coupling of robotic sample processing to matrix-assisted laser desorption time of flight mass spectrometry (MALDI-TOF-MS). Within this par
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Urine Sample Preparation and Protein Profiling by Two-Dimensional Electrophoresis and Matrix-Assisteso considered one of the most difficult proteomic samples to work with due to its highly variable contents, as well as the presence of various proteins in low abundance or modified forms. In this chapter, we describe simple protocols and troubleshooting tips for urinary protein preparation and profi
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Combining Laser Capture Microdissection and Proteomics Techniques cells in several DNA and RNA studies, it has been shown that the small number of cells obtained by this technique can also be used for proteomics analysis. Combining laser capture microdissection and different types of mass spectrometers opened ways to find and identify proteins that are specific f
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Comparison of Protein Expression by Isotope-Coded Affinity Tag Labelingrotein abundance levels. This chapter describes the ICAT labeling procedure in search for the celecoxib-regulated proteins in a colon cancer cell line. Celecoxib, a cyclooxygenase-2 (COX-2) specific inhibitor, is used as a colorectal cancer preventative drug in clinical trials. Here, celecoxib is us
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