Titlebook: Circular RNAs; Methods and Protocol Christoph Dieterich,Argyris Papantonis Book 2018 Springer Science+Business Media, LLC, part of Springer

CLOG

书目名称Circular RNAs影响因子(影响力)




书目名称Circular RNAs影响因子(影响力)学科排名




书目名称Circular RNAs网络公开度




书目名称Circular RNAs网络公开度学科排名




书目名称Circular RNAs被引频次




书目名称Circular RNAs被引频次学科排名




书目名称Circular RNAs年度引用




书目名称Circular RNAs年度引用学科排名




书目名称Circular RNAs读者反馈




书目名称Circular RNAs读者反馈学科排名

hallow

978-1-4939-8521-0Springer Science+Business Media, LLC, part of Springer Nature 2018

Alopecia-Areata

Circular RNAs978-1-4939-7562-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

有权威

Infirm

斜

https://doi.org/10.1007/978-3-642-54508-5t the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.

斜

Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR,plice site, being the unique sequence element of a circular RNA, enables the detection of circular RNA expression. Here we describe the basic techniques to detect different circular transcripts of a gene or one circular RNA specifically by PCR and highlight the advantages and drawbacks of both.

渗透

Nonradioactive Northern Blot of circRNAs,y to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (.P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe to detect circRNA.

mortgage

Preparation of Circular RNA In Vitro,t the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.

Affirm

Christoph Dieterich,Argyris PapantonisIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts