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Titlebook: Circadian Clocks; Tsuyoshi Hirota,Megumi Hatori,Satchidananda Panda Book 2022 The Editor(s) (if applicable) and The Author(s), under exclu

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Regional Anesthesia in Ophthalmologymethods that include loose, cell attached, whole cell, and perforated patch. Also discussed are how particular features of the ipRGC light response affect the design and interpretation of experiments. These approaches may be useful in the broader effort to understand how a neuron’s functional properties align with its role in the organism.
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Studies in Ecological Economicssimple method to prepare and quantitatively measure proteomes and phosphoproteomes in a highly parallelizable manner. We further discuss key factors to be considered when designing time course experiments and during data analysis with the aim to accurately identify circadian and sleep-driven oscillations in proteome and phosphoproteome.
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Studies in Ecological Economicstracing using firefly luciferase as a reporter of circadian gene expression. These methods can be used to study the impact of both central and peripheral clocks on metabolism from various biological samples.
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H. J. Graaf,C. J. M. Musters,W. J. Ter Keursircadian rhythm. Methods presented in this book chapter allow for control of the circadian period in cells, ex vivo and in a living organism, and for the first time enable the modulation of the circadian phase.
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Patch-Clamp Electrophysiological Analysis of Murine Melanopsin Neurons,methods that include loose, cell attached, whole cell, and perforated patch. Also discussed are how particular features of the ipRGC light response affect the design and interpretation of experiments. These approaches may be useful in the broader effort to understand how a neuron’s functional properties align with its role in the organism.
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