Titlebook: Chromosome Painting; Principles, Strategi Arun Kumar Sharma,Archana Sharma Book 2001 Springer Science+Business Media Dordrecht 2001 Chromos

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https://doi.org/10.1057/9780230504592s also given new insights into somaclonal variation, the origin of B chromosomes, control of chromosome pairing and other aspects of chromosome evolution. This review highlights some of these areas and provides source material for the reader who seeks more information on a specific field.

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Book 2001aid of specific probes in molecular hybridization. Its resolution ranges from single copy to entire genome sequences. It is now applied in plant, animal, and human systems, in gene mapping, identification of genetic disorders, evolutionary studies, and gene transfer experiments. This treatise is the

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Chromosome painting in farm, pet and wild animal species,pared to others. The comparisons provide insight into the likely constitution of a vertebrate/mammalian ancestral karyotype and help understand some of the intricacies about karyotype evolution. Importance of comparative painting in setting the stage for rapid development of gene maps in a number of

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Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS,ent centrifugation and/or flow sorting. The methods result in clear signals with negligible non-specific labelling. Further work is needed to improve the sensitivity to allow for reliable detection of single-copy DNA sequences.

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Fluorescent , hybridization in plant polytene chromosomes,lant polytenics, seem to be the most important hindrance for a precise chromosome mapping. On the other hand, the interphase polytene nucleus is a valuable system for localizing FISH signals since they conserve a spatial organization similar to that of mitotic interphase and produce much amplified s

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High resolution FISH to delineate contiguous and small DNA sequences,ridization incubation and post hybridization stringency washing facilitates spatial resolution of contiguous repetitive rye probes pSc 200 and pSc 250, and physical localisation of small RFLP probe xpr 115 of wheat on barley chromosomes.