Titlebook: Chromatin Protocols; Peter B. Becker Book 19991st edition Springer Science+Business Media New York 1999

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Photolyase,hromatin structures in vivo, alternative approaches are applied, such as expression of prokaryotic methyltransferases in ., the genome of which contains no endogenous detectable methylation (.,.). The sites of methylation can be measured after DNA isolation using methylation-sensitive restriction en

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Transcriptional and Structural Analyses of Isolated SV40 Chromatin,ro reconstituted chromatin. SV40 MCs are especially useful in approaching questions regarding stages in transcriptional activation from a potentially competent to a fully active state. Furthermore, because of the utility of SV40 as a viral vector for exogenous promoters (.,.), fully functional cellu

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Book 19991st editiongulators increase transcription by acetylating nucleosomes has lent new support to the old idea that the process of gene regulation is intimately related to the nature of the chromatin environment. A wealth of nonhistone proteins contribute to a continuum of structures with distinct biochemical prop

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Quantitative Analysis of Chromatin Higher-Order Organization Using Agarose Gel Electrophoresis,

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Preparation and Analysis of Positioned Nucleosomes,regulatory transcription factors to nucleosomes (.) and mechanism of transcription of nucleosomal templates (.). The major advantage of this experimental system is relative simplicity of isolation and analysis of uniquely positioned cores.

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Site-Directed Chemical Probing of Histone-DNA Interactions,ticipate in the efficient and controlled utilization of the DNA for nuclear processes such as replication, transcription, recombination, and DNA repair (.). Understanding these complex functions will require a molecular description of the multitude of protein-DNA interactions and associations within