Titlebook: Chromatin Immunoprecipitation; Methods and Protocol Neus Visa,Antonio Jordán-Pla Book 2018 Springer Science+Business Media LLC 2018 ChIP as

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https://doi.org/10.1007/978-1-4020-6460-9al intracellular processes such as DNA replication, transcription regulation, chromatin stability, and others are all dependent on protein interactions with DNA. The DNA fragments enriched from the ChIP assay are analyzed by downstream applications, for example, microarray hybridization (ChIP-chip),

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https://doi.org/10.1007/978-1-4020-6460-9cription factor binding sites and histone chemical modifications. However, these approaches only allow profiling of a single factor or protein modification at a time..In this chapter, we present Bar-ChIP, a higher throughput version of ChIP-Seq that relies on the direct ligation of molecular barcode

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Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experimentteins and genomic DNA. Ever since its inception, more than 30 years ago, ChIP has been constantly evolving, improving, and expanding its capabilities and reach. Despite its widespread use by many laboratories across a wide variety of disciplines, ChIP assays can be sometimes challenging to design, a

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