Titlebook: Chemosensitivity; Volume I: In Vitro A Rosalyn D. Blumenthal Book 2005 Humana Press 2005 cell.cell culture.cell death.chemotherapy.imaging.

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Advanced Parallel Processing Technologiesombination than expected from the sum of each agent alone). Here, we briefly review some of the principles for testing cytotoxic drug interactions. We focus this review on application of the Combination Index method (as developed by Chou and colleagues) in the evaluation of drug interactions in cell culture assays.

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Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity,een malignant and contaminating nonmalignant cells. The latter is a major advantage of the DiSC assay. This chapter describes the practical aspects of this assay, several topics that need to be taken into account, and potential pitfalls. As such, it is not an extensive review of studies in which the DiSC assay was used.

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Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity,number of cells for the test, easy quantification of the anticancer effects without contamination with fibroblasts by using an image analysis system, a good correlation between in vitro and in vivo results, and simplicity and speed. The CD-DST method can be performed in the laboratory using a system kit Primaster®.

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In Vitro Testing of Chemosensitivity in Physiological Hypoxia,oved by conducting testing in conditions of physiological hypoxia. We review the impact of hypoxia on anticancer drug cytotoxicity and the methods used in our laboratory to asses the cytotoxic activity of single antineoplastic drugs under conditions of physiological hypoxia.

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1543-1894 vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In additi

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Sulforhodamine B Assay and Chemosensitivity,assays such as MTT or clonogenic assay. The SRB assay possesses a colorimetric end point and is nondestructive and indefinitely stable. These practical advances make the SRB assay an appropriate and sensitive assay to measure drug-induced cytotoxicity even at large-scale application.