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Titlebook: Chemokine-Glycosaminoglycan Interactions; Methods and Protocol Alexandra R. Lucas Book 2023 The Editor(s) (if applicable) and The Author(s)

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发表于 2025-3-21 16:08:07 | 显示全部楼层 |阅读模式
书目名称Chemokine-Glycosaminoglycan Interactions
副标题Methods and Protocol
编辑Alexandra R. Lucas
视频videohttp://file.papertrans.cn/225/224911/224911.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chemokine-Glycosaminoglycan Interactions; Methods and Protocol Alexandra R. Lucas Book 2023 The Editor(s) (if applicable) and The Author(s)
描述This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Chemokine-Glycosaminoglycan Interactions: Methods and Protocols. serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro..
出版日期Book 2023
关键词Immune cell trafficking; Immune responses; Autoimmune disorders; GAG networks; Organ and tissue models; P
版次1
doihttps://doi.org/10.1007/978-1-0716-2835-5
isbn_softcover978-1-0716-2837-9
isbn_ebook978-1-0716-2835-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Remote Ischemic Pre-Conditioning (RIPC),linic. Many researchers have tried to introduce brief I/R in skeletal muscle to mimic cardiac ischemic pre-conditioning, called remote ischemia pre-conditioning (RIPC). Studies from our group and other groups have shown that RIPC induces the release of cytokines from skeletal muscle (myokines) for t
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Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion,transwell or flow chamber system. Here we describe a more physiologically relevant, sophisticated, and highly flexible method to study leukocyte adhesion ex vivo in fresh murine carotid arteries under arterial flow conditions. This model mimics an in vivo situation and allows the combination of leuk
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In Vitro (Trans)Migration Experiment Using Chemokines as Stimulatory Factor,ssical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier, which cells have to overcome by active migration. A large variety of Boyden chambers are available and can be customized to fit the experiment by choosing pore size, density, and membrane type
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Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cellllowing these proteins to be anchored at the cell surface to increase their anti-chemokine activity in the proximity of infection. Here we describe methodology to evaluate the interaction of viral secreted proteins with cell-surface glycosaminoglycans by immunofluorescence and detection by flow cyto
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