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Titlebook: Cellulases; Methods and Protocol Mette Lübeck Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 biomass.lignocel

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Gliederung eines Operationsberichtesincreased in the recent years. In this chapter, methods for discovery of novel cellulosomal cellulases from a DNA sequence by bioinformatics and biochemical tools are described. Their biochemical characterization is also described, including both the enzymatic activity of the putative cellulases and
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https://doi.org/10.1007/978-3-642-20790-7designer cellulosomes, composed of mixtures of mesophilic and thermophilic components, have been constructed. As a result, the overall thermal profile of the individual parts and the resulting complex has to be extensively evaluated. Here, we describe a practical guide for the determination of tempe
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https://doi.org/10.1007/978-3-642-21195-9tion in substrate and quantification of hydrolysates. However, the Avicel method is still considered as the most promising and efficient hydrolytic technique so far. The most commonly used CBH assays including Avicel and other recent methods for proper quantification are outlined in this chapter. Al
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A Dynamic Optimization Framework for OpenMPns, in particular the site of oxidation. Kinetic studies of LPMOs have several pitfalls and to avoid these, it is important to secure copper saturation, avoid the presence of free transition metals in solution, and control the amount of reductant (i.e., electron supply to the LPMO). Further insight
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https://doi.org/10.1007/978-1-4939-7877-9biomass; lignocellulose; biorefining; archea; enzyme production
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