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Titlebook: Cellular Heterogeneity; Methods and Protocol Natasha S. Barteneva,Ivan A. Vorobjev Book 2018 Springer Science+Business Media, LLC 2018 Live

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发表于 2025-3-21 19:02:24 | 显示全部楼层 |阅读模式
书目名称Cellular Heterogeneity
副标题Methods and Protocol
编辑Natasha S. Barteneva,Ivan A. Vorobjev
视频video
概述Includes cutting-edge techniques for the study of cellular heterogeneity.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cellular Heterogeneity; Methods and Protocol Natasha S. Barteneva,Ivan A. Vorobjev Book 2018 Springer Science+Business Media, LLC 2018 Live
描述This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. .Authoritative and accessible, .Cellular Heterogeneity: Methods and Protocols. encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries..
出版日期Book 2018
关键词Live cell imaging; Cytometric data; Cell population; 3D cell cultures; Flow cytometry; Heterogeneity trac
版次1
doihttps://doi.org/10.1007/978-1-4939-7680-5
isbn_softcover978-1-4939-8541-8
isbn_ebook978-1-4939-7680-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2018
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Organisation und Welterschließungphages represent heterogeneous populations, which can modulate CNS environment and have different effects on neuronal regeneration. In this chapter, the main features of microglial and macrophage subsets and current methods for investigation of their heterogeneity will be discussed.
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Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Centraphages represent heterogeneous populations, which can modulate CNS environment and have different effects on neuronal regeneration. In this chapter, the main features of microglial and macrophage subsets and current methods for investigation of their heterogeneity will be discussed.
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Multiparametric Analysis of Myeloid Populations by Flow Cytometryining, and analysis, which include a newly described viSNE tool to generate two-dimensional clustering within myeloid populations. We also reference the use of transgenic reporter mice on specific myeloid cells that provides enhanced specificity and profiling when defining myeloid heterogeneity.
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Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoaell populations of protozoan parasites using a combination of nested PCR amplification and capillary electrophoresis. With this workflow, in silico gels from isolated cell clones can be generated, thus providing an excellent tool for analyzing cellular heterogeneity in protozoan parasites.
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