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Titlebook: Cell-Free Translation Systems; Alexander S. Spirin Book 2002 Springer-Verlag Berlin Heidelberg 2002 Expression.Polymerasekettenreaktion.Pr

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Miriam Adebibe,Simon W. Coppack also papers in Roberts 1958). The protein-synthesizing ability of these particles was experimentally proved (Littlefield et al. 1955; Littlefield and Keller 1957; McQuillen et al. 1959). The word “ribosome”, was proposed to designate the protein-synthesizing ribonucleoprotein particles.
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Yeast Cell-Free Translation Systemsor activity through various means, including downregulation of factor synthesis, expression of conditional lethal factor or knock-out mutants and inhibition of factor activity by antibodies. Such systems have been used to extend and deepen our knowledge of the mechanism of translation initiation, protein targeting and translation termination.
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Symmetric and Isometric Relations system. The PURE system produced active enzymes without the aid of molecular chaperones. The PURE system that is reinforced with trans-translation system shows increased productivity and addition of human placental RNase inhibitor also resulted in prolonged production time.
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ICU Staffing, Models, and Outcomes. However, if aggregation also occurs in cell-free systems, the conditions can easily be adjusted to overcome the problem. For example, the addition of detergents, cofactors, or molecular chaperones can often enhance or achieve protein solubility.
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