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Titlebook: Cell Viability Assays; Methods and Protocol Oliver Friedrich,Daniel F. Gilbert Book 2023Latest edition The Editor(s) (if applicable) and Th

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发表于 2025-3-21 19:11:19 | 显示全部楼层 |阅读模式
书目名称Cell Viability Assays
副标题Methods and Protocol
编辑Oliver Friedrich,Daniel F. Gilbert
视频videohttp://file.papertrans.cn/223/222896/222896.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell Viability Assays; Methods and Protocol Oliver Friedrich,Daniel F. Gilbert Book 2023Latest edition The Editor(s) (if applicable) and Th
描述This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Cell Viability Assays: Methods and Protocols, Second Edition. serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environmen
出版日期Book 2023Latest edition
关键词Cell fitness; Physiological and patho-physiological stress; 2D environments; 3D assays; Cell function; Bi
版次2
doihttps://doi.org/10.1007/978-1-0716-3052-5
isbn_softcover978-1-0716-3054-9
isbn_ebook978-1-0716-3052-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions Common in vitro culture models are typically maintained under static conditions, where the surrounding culture medium is replaced every few days—typically every 48 to 72 h—with the aim to remove metabolites and to replenish nutrients. Although this approach is sufficient for supporting cellular sur
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Assaying Mitochondrial Function by Multiparametric Flow Cytometryhemical attributes of individual cells within a larger population. More recently, advances in flow cytometry have enabled nanoparticle detection. This is particularly applicable to mitochondria, which, as intracellular organelles have distinct subpopulations that can be evaluated based on difference
Preserve
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Average Rheological Quantities of Cells in Monolayersgether in a single layer. Here we describe step-by-step procedure as to how to employ a modified commercial rotational rheometer to run rheological measurement and detect average viscoelastic properties of cells while maintaining the necessary precision level at the same time.
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Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arraysmine chemical effects thereon. Following assessment of compound effects on multiple endpoints that evaluate network function, a cell viability endpoint in the same well is determined using a multiplexed approach. Recently, it has become possible to measure electrical impedance of cells attached to t
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