Titlebook: Cell Tracking; Methods and Protocol Matthew T Basel,Stefan H Bossmann Book 2020 Springer Science+Business Media, LLC, part of Springer Natu

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1064-3745 ation advice from the experts.This volume details methods used to track cells in the body and will serve as a reference for preclinical and clinical researchers in the fields of medicine and biomedical science.  Chapters guide readers through protocols on bioluminescence imaging, fluorescence imagin

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https://doi.org/10.1007/BFb0051459s, including fluorescent protein and luciferase, but both have limitations. Here, we introduce a protocol using iRFP genetic labeling technology to track tumor formation of iPSCs in skeletal muscle after CRISPR/Cas9 gene editing.

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Conference proceedings 19661st editionods Mol Biol 750:79–90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells.

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Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo,tem cell survival and retention, making it a powerful tool to monitor cell fate after transplantation without animal sacrifice. Here we describe a protocol that allows luciferase-labeled stem cells to be imaged and tracked in vivo by bioluminescent imaging via an IVIS spectrum imaging system.

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MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles,ods Mol Biol 750:79–90, 2011). Thus, in this chapter, we will mainly focus on the tracking of SPIO-nanoparticle-labeled mouse dendritic cells by MRI and provide a detailed protocol for cell labeling and in vivo tracking by a clinical 3.0T MRI scanner. Of note, this protocol is also suitable to be applied on other types of cells.

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