Titlebook: Cell Reprogramming for Immunotherapy; Methods and Protocol Samuel G. Katz,Peter M. Rabinovich Book 2020 Springer Science+Business Media, LL

Arbitrary

Microfluidic Approach for Highly Efficient Viral Transductionfluidic devices for efficient ex vivo gene transfer. Up to four- to fivefold reductions in viral vector usage and two- to fourfold reductions in transduction times can be obtained by using this method.

CLOWN

Single-Cell Cytokine Analysis to Characterize CAR-T Cell Activationo study their heterogeneous cytokine response at the single-cell level following activation. Here, we describe an activation platform which incubates single CAR-T cells with single-target CD19 antigen-presenting cells (APCs) and measures the resulting cytokine secretions. This can be compared to the

大约冬季

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Minikin

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cellsnthetic” genes. NK cells represent 10–20% of total lymphocytes in the peripheral blood of humans and play an essential role in clearing infections and malignant cells. A significant number of NK cells express and utilize non-clonotypic receptors that recognize cognate ligands expressed on a broad sp

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OCTO

Immunomodulation of NK Cell Activity modulate the activity of different cells of the immune system. The recognition of target cells is mediated by germ line-encoded receptors, and the activity of NK cells can be further regulated by soluble factors such as cytokines and Toll-like receptor ligands. Thus, NK cells display an exciting po

绅士

An Overview of Advances in Cell-Based Cancer Immunotherapies Based on the Multiple Immune-Cancer Celsystemic levels. A number of studies indicate that various immune cells participate in tumor development (Fig. .). In this review, we will discuss interactions among T lymphocytes (T cells), B cells, natural killer (NK) cells, dendritic cells (DCs), tumor-associated macrophages (TAMs), neutrophils,

EWE

Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapyarrow precursors or human blood monocytes to DC via cultivation with supraphysiologic concentrations of cytokines such as GM-CSF and IL-4 for up to 7 days. Alternatively, our group has recently established a new approach, based on the underlying mechanism of action of a widely used cancer immunother