Titlebook: Cell Cycle Control and Dysregulation Protocols; Antonio Giordano,Gaetano Romano Book 2004 Humana Press 2004

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Immunofluorescence Analysis Using Epitope-Tagged Proteins proteins (green fluorescent protein [GFP], cyan fluorescent protein, yellow fluorescent protein, and red fluorescent protein). Caution must be exercised when tagging large proteins (e.g., GFP or β-galactosidase) at the amino or carboxy termini to avoid interference with folding that can influence a

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Chromatin Immunoprecipitationosomes. Formaldehyde crosslinking rapidly fixes protein-protein and protein-DNA complexes in vivo providing the basis for an approach to analyze native structure of gene promoters. The protocol below is a simple yet sensitive method to determine whether a known protein is associated with a DNA seque

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Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expressionences would decrease the band intensity of the previously shifted complexes, whereas mutated or unrelated binding sequences will not change the intensity of the previously shifted complexes (an example is shown in .).

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A Morphologic Approach to Detect Apoptosis Based on Electron Microscopyarply circumscribed, uniformly-dense masses that abut on the nuclear envelope and condensation of the cytoplasm. Continuation of condensation is accompanied by convolution of the nuclear and cellular outlines, and nucleus often break up at this stage to produce discrete fragments. The surface protub

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Book 2004fident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle. We greatly acknowledge the extraordinary contribution of the authors in writing this book.

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