Titlebook: Cell Cycle Control and Dysregulation Protocols; Antonio Giordano,Gaetano Romano Book 2004 Humana Press 2004

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Protein Degradation Via the Proteosomel cycle (.). The importance of protein degradation in the control of cell proliferation is elegantly illustrated by the regulated synthesis and destruction of the cyclin family of proteins during the life cycle of a cell. The cyclins are a group of proteins that influence the function of a series of

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Induction of Deoxyribonucleic Acid Damage by Alkylating Agentsr electrophilic nature, alkylating agents have an affinity for the nucleophilic centers of DNA and are monofunctional (have one reactive group) or bifunctional (have two reactive groups). Alkylating agents, such as methylmethanesulphonate and .-ethyl-.-nitrosourea, introduce methyl, ethyl, and compl

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1064-3745 e, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Becaus

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The Essence of Acts of Empathy,tly labeled antibodies. Procedures for BrUTP of labeling transcription sites require manipulations that are best applied to adherent cells but can be applied, with difficulty, to cell cultures in suspension. The protocol described below is for adherent cells grown on cover slips.

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https://doi.org/10.1007/978-94-017-6067-6o occupancy of gene regulatory elements by LM-PCR largely depends on the strategy and quality of reagents. The experimental outline for LM-PCR reaction is shown in .. For a through understanding of this procedure and other alternative options, . Ausubel et al. (.).