Titlebook: Cell Cycle Checkpoints; Methods and Protocol James J. Manfredi Book 2021 The Editor(s) (if applicable) and The Author(s) 2021 Flow cytometr

reptile

Kees B. Geerse,Jan C. M. Marijnissen as microtubular poisons or actin blockers. The protocol described herein details a method to quantify the phosphorylation of eIF2α by high-throughput immunofluorescence microscopy. This method is useful to capture the ‘integrated stress response’, which is characterized by eIF2α phosphorylation in the context of mitotic catastrophe.

compel

1064-3745 ation advice from the expertsThis volume explores the latest advancements in the field of cell cycle checkpoints and their implications for human diseases. Chapters in this book cover topics such as post-translationally modified p53 by western blotting; CHK1 cellular localization by immunofluorescen

护身符

The Puzzling Case of Alterity in Husserl’s ,ventional method for studying the cellular localization of CHK1, a cell cycle checkpoint kinase that undergoes shuttling from the cytoplasm to the nucleus in response to genotoxic stress. In this study, we included an acid extraction step to better reveal the nuclear localization of CHK1.