Titlebook: Cell Cycle Checkpoint Control Protocols; Howard B. Lieberman Book 2004 Humana Press 2004

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The Analogical Inference to Other Minds,equired for S phase depends on the size of the genome, the organism, and its developmental state. DNA replication requires only 15 to 20 min in budding yeast, but 6 to 7 h in mammalian cells. In organisms, such as frogs, fish, echinoderms, and flies that undergo rapid cell cleavage events at the beg

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Wim J. M. Dekkers,Henk A. M. J. ten Havethod than in carrying out the work. Thus, this chapter concentrates more on the alternative strategies and under which circumstances the choice of a given method is appropriate. There are essentially three types of strategies for isolating conserved genes of any sort: through deoxyribonucleic acid (

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Wim J. M. Dekkers,Henk A. M. J. ten Havethe genetic analysis of mutations has helped reveal the normal function of wild-type gene products as well as provided powerful insights into the intricate relationships between large numbers of gene products. The use of genetic screens and straightforward selection techniques in simple eukaryotes,

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Sunil Kumar Agrawal,Brian C. Fabienample, a number of proteins that are necessary for responding to deoxyribonucleic acid (DNA) damage were found in screens for loss-of-function mutants that confer hypersensitivity to DNA-damaging agents (.). Genetic screens that report a loss of function can also be used to discern structure-functio

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Optimization of Dynamic Systemslatory proteins that function during the transition between mitosis and meiosis have extensively relied on targeted in vitro mutagenesis coupled with in vivo genetic assays (.,.). For example, fission yeast Mei3p is a meiotic activator. It functions by binding to and inhibiting a protein kinase that