Titlebook: Cell Culture Engineering IV; Improvements of Huma Barry C. Buckland,John G. Aunins,Weichang Zhou Book 1995 Springer Science+Business Media

Devastate

Adaptation of mammalian cells to non-ammoniagenic media,ted through storage or by cellular metabolism, leading to the formation of ammonia which can result in growth inhibition. Non-ammoniagenic alternatives to glutamine have been investigated in an attempt to develop strategies for obtaining improved cell yields for ammonia sensitive cell lines..Glutama

背叛者

Veneer

Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuousl growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage.

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食料

Offbeat

Recombinant protein expression in a , cell line: comparison with the baculovirus system,t S2 cell line is simple and quick, and in batch fermentations the cells have a doubling time of 20 hours until reaching a plateau density of 20 million cells/ml. Protein expression is driven by the . Metaliothionein promoter which is tightly regulated. When expressed in S2 cells, the extracellular

debouch

Scale-up of the adenovirus expression system for the production of recombinant protein in human 293new constructions of adenovirus vectors, yielded as much as 10 to 20% recombinant protein with respect to the total cellular protein content. Until recently, high specific productivity of recombinant protein was limited to low cell density infected cultures of no more than 5 × 10. cells/mL. In this

Innovative

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