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Titlebook: Cartilage and Osteoarthritis; Massimo Sabatini,Philippe Pastoureau,Frédéric Ceun Book 2004 Humana Press 2004

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In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors,n, the use, and the effect of the transduction of chondrocytes or synovial fibroblasts with an adenoviral vector encoding the cDNA for glutamine: fructose-6-phosphate amidotransferase (GFAT). This is intended to serve as an example of a technology that can be used to evaluate the biological effects of overexpression of other cDNAs.
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Davide Taibi,Luigi Lavazza,Sandro Morascan this way, chondrocytes can be recovered and further separated from the matrix by centrifugation. Almost all molecular and biochemical techniques, as well as a number of biological assays, are compatible with the culture of chondrocytes in alginate.
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https://doi.org/10.1007/978-0-387-72486-7g any immortalizing agent. Although immortalized chondrocytes should not be considered as substitutes for primary chondrocytes, they may be useful tools for evaluating and further validating mechanisms relevant to cartilage biology.
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Culture of Chondrocytes in Alginate Beads,n this way, chondrocytes can be recovered and further separated from the matrix by centrifugation. Almost all molecular and biochemical techniques, as well as a number of biological assays, are compatible with the culture of chondrocytes in alginate.
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Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Linesg any immortalizing agent. Although immortalized chondrocytes should not be considered as substitutes for primary chondrocytes, they may be useful tools for evaluating and further validating mechanisms relevant to cartilage biology.
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Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chonds only one cell type in both normal and diseased conditions. However, care has to be taken not to over- and misinterpret results, and some major challenges must be overcome in order to utilize the potential of this technology properly in the field of osteoarthritis.
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Changes of Chondrocyte Metabolism In Vitro,rypsin and matrix-assisted laser desorption ionization-time of flight mass spectrometry, using peptide mass fingerprinting. Tryptic peptide masses were measured and matched against a computer-generated list from the simulated trypsin proteolysis of a protein database (SwissProt).
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