Titlebook: Cardiomyocytes; Methods and Protocol Gary R. Skuse,Maureen C. Ferran Book 2015 Springer Science+Business Media New York 2015 Cardiac myocyt

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Cryopreservation of Neonatal Cardiomyocytes,oliferative abilities, must be performed frequently. To reduce the time requirements and the impact on research animals, we describe a method for cryopreserving neonatal rat cardiomyocytes (NRCMs), and subsequently thawing them for use in assays.

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Gary R. Skuse,Maureen C. FerranIncludes cutting-edge methods and protocols in cardiomyocyte research.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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James Chesko,Thomas Vedvick,Steve Reedtain because they do not proliferate after birth. The maintenance of primary cultures of viable and functional cardiomyocytes is considerably affected by the yield from initial steps of isolation procedures. This protocol describes an efficient and rapid method for isolation and maintenance of long-

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Ankur Singh,Pallab Pradhan,Krishnendu Royart to stress stimuli. As the sequence-specific recognition element of RNA-induced silencing complexes (RISCs), microRNAs bind mRNAs and prevent their translation via mechanisms that may include transcript degradation and/or prevention of ribosome binding. Short microRNA sequences and the ability of

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Parminder Singh,Guohua Chen,Wade Worshamsible way for obtaining global genomic information. At present, three most NGS platforms are used in genetics for clonally amplified templates. These technologies share general processing steps but differing in specific technical details that determine their limits or advantages. NGS has been recent