Titlebook: Cardiac Regeneration; Methods and Protocol Kenneth D. Poss,Bernhard Kühn Book 2021 Springer Science+Business Media, LLC, part of Springer N

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Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration tes. Moreover, we describe a cardiac differentiation protocol to derive cardiomyocytes from hiPSCs, as well as a strategy to use fluorescence-activated cell sorting to isolate pure populations of fluorescently labeled cardiomyocytes for downstream applications.

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https://doi.org/10.1007/978-1-349-02402-5grade intracoronary application of locally acting pharmaceutical agents, viruses, and cells. Here, we describe the techniques of left ventricular catheterization and induction of ischemia and reperfusion, as well as hemodynamic monitoring and regional application of therapeutic agents in pigs.

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1064-3745 expertsThis detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac inju

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https://doi.org/10.1007/978-3-319-47042-9bility and reliability of the results obtained. Here, we describe the technique of left ventricular catheterization using the Scisense conductance catheter system by Transonic; however, the basic method applies to all murine catheter systems. We furthermore indicate possible pitfalls during the procedure and how to avoid them.

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https://doi.org/10.1007/978-1-349-02402-5 been developed in zebrafish, including ventricular resection, genetic ablation, and ventricular cryoinjury. This chapter provides a detailed protocol of zebrafish ventricular cryoinjury and highlights factors and critical steps to be considered when performing this method.

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Left Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial bility and reliability of the results obtained. Here, we describe the technique of left ventricular catheterization using the Scisense conductance catheter system by Transonic; however, the basic method applies to all murine catheter systems. We furthermore indicate possible pitfalls during the procedure and how to avoid them.