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Titlebook: Cardiac Gene Expression; Methods and Protocol Jun Zhang,Gregg Rokosh Book 2007 Humana Press 2007 DNA.In silico.Microarray.PCR.Polymerase Ch

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书目名称Cardiac Gene Expression
副标题Methods and Protocol
编辑Jun Zhang,Gregg Rokosh
视频video
概述Resources for the analysis of gene regulation data and SNPs.Comparative genomic approach for functionally annotating human DNA.Quantitative, real-time RT-PCR in cardiovascular research.In silico analy
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cardiac Gene Expression; Methods and Protocol Jun Zhang,Gregg Rokosh Book 2007 Humana Press 2007 DNA.In silico.Microarray.PCR.Polymerase Ch
描述.Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function...Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome
出版日期Book 2007
关键词DNA; In silico; Microarray; PCR; Polymerase Chain Reaction; Promoter; SNP; Single Nucleotide Polymorphism; g
版次1
doihttps://doi.org/10.1007/978-1-59745-030-0
isbn_softcover978-1-61737-514-9
isbn_ebook978-1-59745-030-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2007
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Quantitative (Real-Time) RT-PCR in Cardiovascular Researchtation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples. The sensitivity of this method permits the reliable detection of low concentrations of initial template, while deliverin
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Hybridization rapid methodology provides precious insights into the complex gene organization and expression within an heterogeneous cell population. This technique is particularly useful to elucidate the genes and pathways involved in cardiac cells processes (differentiation, proliferation, apoptosis) or in the
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Characterization of ,-Regulatory Elements and Transcription Factor Binding gel mobility shift assay (GMSA) or gel retardation assay are essential and common steps. In addition to providing a general protocol for GMSA, this chapter describes some applications of this assay to characterize cardiac-specific and ubiquitous .-acting factors bound to regulatory elements [novel
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