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Titlebook: Capillary Electrophoresis of Proteins and Peptides; Mark A. Strege,Avinash L. Lagu Book 2004 Humana Press 2004

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楼主: Strategy
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Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection,olumn-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.
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Biclustering of Time Series Microarray Datas binding at the indole-benzodiazepine site. By comparing the mobility of a solute in the presence of these two modified forms of HSA vs normal HSA, it is possible to detect solute interactions at these binding sites. This approach is illustrated using warfarin and ibuprofen as examples of test solutes.
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Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis,cationic and anionic proteins. The electroosmotic flow (EOF) is strongly suppressed in DLPC-coated capillaries, thus low mobility proteins are slow to elute, and so the coating is favored for separation of high mobility proteins.
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On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electxtract from cultured cells, and (4) manipulations associated with analyses of proteins in single cells. More than 20 relevant publications are cited in this chapter to assist the reader with adopting the presented methods.
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Capillary Isoelectric Focusing-Mass Spectrometry of Proteins and Protein Complexes,-cell lysate for the CIEF separation and MS detection on the intact protein level. The protocol was further adapted for the analysis of the mixture of noncovalent complexes on the intact complex level.
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Md. Ahsan,P. Chand,Kumari Namratacationic and anionic proteins. The electroosmotic flow (EOF) is strongly suppressed in DLPC-coated capillaries, thus low mobility proteins are slow to elute, and so the coating is favored for separation of high mobility proteins.
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