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Titlebook: Cancer Immunotherapy; Methods and Protocol Velia Siciliano,Francesca Ceroni Book 2024 The Editor(s) (if applicable) and The Author(s), unde

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https://doi.org/10.1007/1-4020-4812-2Retroviral transduction is a highly useful tool to genetically engineer hard-to-transfect human primary cells. Here, we transduce human primary T cells with a tumor-specific T cell receptor. This creates a useful tool to analyze T cell–cancer cell interactions, such as cytolysis analysis using xCELLigence technology.
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https://doi.org/10.1007/978-981-10-3193-9related to basic functional and phenotypic analysis are described. This methodology can be applied to manufacture and assess chimeric antigen receptors for preclinical applications targeting a variety of molecules.
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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221137.jpg
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A New Microfluidic Device to Facilitate Functional Precision Medicine Assays,itations such as the number of cells and trained personnel required. To overcome these impediments, here we describe a novel microfluidic platform that can be used to perform FPM assays, optimizing the use of primary cancer cells and simplifying the process by using microfluidics to automatize the process.
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1064-3745 ng-edge, .Cancer Immunotherapy: Methods and Protocols .aims to ensure successful results in the further study of this vital field..978-1-0716-3595-7978-1-0716-3593-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
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