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Titlebook: Cancer Genomics and Proteomics; Methods and Protocol Paul B. Fisher Book 2007 Humana Press 2007 BAC.Carcinom.Chromosom.DNA.Microarray.Trans

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发表于 2025-3-21 18:50:53 | 显示全部楼层 |阅读模式
书目名称Cancer Genomics and Proteomics
副标题Methods and Protocol
编辑Paul B. Fisher
视频videohttp://file.papertrans.cn/222/221118/221118.mp4
概述A compendium of techniques and applications in gene identification and function.Approaches described in detail are state-of-the art and can be tailored to individual ongoing or planned research projec
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cancer Genomics and Proteomics; Methods and Protocol Paul B. Fisher Book 2007 Humana Press 2007 BAC.Carcinom.Chromosom.DNA.Microarray.Trans
描述.Cancer Genomics and Proteomics: Methods and Protocols provides a compendium of techniques and applications that will be of profound use to researchers interested in gene identification and function. The approaches described in this volume are state-of-the-art and may be tailored to ongoing individual or planned research projects. ..Reviews are written by experts in specific aspects of gene identification and full-length gene cloning, gene profiling (microarrays), chromatin modification of gene regulation, bacterial artificial chromosomes, cancer cytogenetic analyses, and gene methylation. Chapters also discuss topics such as phage display, yeast and mammalian two-hybrid systems, RNA silencing, monoclonal antibody production, kinases and signal transduction, PKR, analyses of mouse embryo fibroblasts, protein microarrays, and protein crystallization. ..Cancer Genomics and Proteomics: Methods and Protocols will be of interest to molecular biologists, geneticists, cell biologists, and biochemists involved in studying genes associated with and regulating important physiological processes. This volume will serve as a valuable laboratory resource for designing experiments to identify and
出版日期Book 2007
关键词BAC; Carcinom; Chromosom; DNA; Microarray; Translation; gene expression; genes; hybridization; signal transdu
版次1
doihttps://doi.org/10.1007/978-1-59745-335-6
isbn_softcover978-1-61737-605-4
isbn_ebook978-1-59745-335-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2007
The information of publication is updating

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Cloning Differentially Expressed Genes Using Rapid Subtraction Hybridization (RaSH), differentially expressed genes represent critical elements in this process. Many techniques have been developed to facilitate achieving these objectives. Although effective in many situations, most currently described approaches are not trouble-free and have limitations, including complexity of per
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The Application of Differential Display as a Gene Profiling Tool,fications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our l
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Gene Expression Profile Analysis of Tumors,les for all known genes in the human genome. The genome-wide analysis of the gene expression patterns of neoplastic and normal cells provides insights into: (1) the identification of previously unknown tumor subtypes; (2) the normal cellular counterparts of tumor cells; (3) the identification of cel
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Complete Open Reading Frame (C-ORF) Technique,CR. However, these protocols are not infallible and in specific instances they have proven unsuccessful, emphasizing a need for further refinement. A novel method, the complete open reading frame (C-ORF) technique, is presently described, which has proven successful in cases, where standard rapid am
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Chromatin Immunoprecipitation Assays,es, their downstream targets, or owing to increased proliferation, and altered apoptotic potential. Various microarray based techniques have been widely utilized to study the differential expression of genes in cancer in recent years. Along with this, attempts have been made to study the transcripti
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Manipulating Genes and Gene Copy Number by Bacterial Artificial Chromosome Transfection,nd other large genomic loci. Protocols and parameters that influence the efficient transfection of these large DNA molecules into cells in culture were described here. By carefully optimizing the conditions for the formation of compact transfection complexes, BACs can be introduced into a variety of
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