Titlebook: Cancer Driver Genes; Methods and Protocol Timothy K. Starr Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 thr

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Arrayed shRNA Screening to Identify Suppressors of Anchorage-Independent Growthd in 96-well plates. These modifications decrease hands-on time, increase fidelity of the assay, and make it possible to screen 500–1000 short-hairpin RNAs (shRNA) in “one-shRNA-one-well” format in parallel. These protocols can also be used to conduct functional cDNA or CRISPR screens for modulators of anchorage-independent growth.

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CRISPR/Cas9-Based Positive Screens for Cancer-Related Traitsnew example is the application of CRISPR/Cas9-based library screening for cancer-related traits in cell lines. Such screens can reveal genome-wide suppressors of tumorigenesis and metastasis. Here we describe the use of widely available lentiviral libraries for such screens in cultured cell lines.

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Cancer Gene Discovery: Past to Present, death from cancer. Attempts have been made to define the phenotypic and genetic “hallmarks” of cancer, but many of these “hallmarks” remain descriptive, while the underlying mechanisms responsible for these hallmarks remain elusive. For decades, cancer researchers have been methodically identifying

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Identification of Cancer Driver Genes from a Custom Set of Next Generation Sequencing Dataver mutated genes that confer a selective advantage for cancer cells. Numerous computational algorithms have been developed to find genes that drive cancer based on their patterns of mutation in a patient cohort. It has been noted that the distinct features of driver gene alterations in different su

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