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Titlebook: Cancer Cell Culture; Methods and Protocol Simon P. Langdon Book 20041st edition Humana Press 2004

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发表于 2025-3-21 16:07:47 | 显示全部楼层 |阅读模式
书目名称Cancer Cell Culture
副标题Methods and Protocol
编辑Simon P. Langdon
视频videohttp://file.papertrans.cn/222/221061/221061.mp4
概述Includes supplementary material:
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Cancer Cell Culture; Methods and Protocol Simon P. Langdon Book 20041st edition Humana Press 2004
描述The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, ad
出版日期Book 20041st edition
版次1
doihttps://doi.org/10.1385/1592594069
isbn_ebook978-1-59259-406-1Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2004
The information of publication is updating

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Global Neuroethics in Early 2019s and features that are universally applied. This chapter describes some of the essential techniques and provides typical protocols. It is assumed that aseptic technique will be used for all these procedures unless mentioned otherwise.
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https://doi.org/10.1007/978-1-4614-4842-6cases per year and in excess of 12,000 deaths in the United States (.). RCC is rare in the first two decades of life, comprising only 2% of pediatric tumors. The incidence increases with advancing age, peaking in the sixth decade of life.
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Global Neuroethics in Early 2019s and features that are universally applied. This chapter describes some of the essential techniques and provides typical protocols. It is assumed that aseptic technique will be used for all these procedures unless mentioned otherwise.
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Neuroethics and the Regulation of Misusell-line models. Characterization of the properties of cell lines is important for a number of reasons. First, the relationship of the cell line to the cells of origin should be established to confirm that the cell line is derived from and is representative of its tissue of origin. If the cell line i
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https://doi.org/10.1007/978-3-030-53790-6ine could be cultured continuously. The application of specific species markers, including cell-surface antigens and chromosomes, showed that interspecies misidentification was a widespread problem (.,.). Subsequently, it was shown that intraspecies contamination of human cell cultures was also a se
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