书目名称 | Cancer Cell Culture |
副标题 | Methods and Protocol |
编辑 | Simon P. Langdon |
视频video | http://file.papertrans.cn/222/221061/221061.mp4 |
概述 | Includes supplementary material: |
丛书名称 | Methods in Molecular Medicine |
图书封面 |  |
描述 | The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, ad |
出版日期 | Book 20041st edition |
版次 | 1 |
doi | https://doi.org/10.1385/1592594069 |
isbn_ebook | 978-1-59259-406-1Series ISSN 1543-1894 Series E-ISSN 1940-6037 |
issn_series | 1543-1894 |
copyright | Humana Press 2004 |