| 书目名称 | Cancer Cell Culture | | 副标题 | Methods and Protocol | | 编辑 | Ian A. Cree | | 视频video | http://file.papertrans.cn/222/221060/221060.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .With many recent advances, cancer cell culture research is more important than ever before. This timely edition of .Cancer Cell Culture: Methods and Protocols. covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful .Met | | 出版日期 | Book 2011Latest edition | | 关键词 | Cancer Cell Biology; Cancer Cell Isolation; Cancer Cell Lines; Cancer Therapy; Cell Modification; Functio | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-61779-080-5 | | isbn_softcover | 978-1-4939-5657-9 | | isbn_ebook | 978-1-61779-080-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2011 |
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