Titlebook: Cancer Biomarkers; Methods and Protocol Gagan Deep Book 2022 Springer Science+Business Media, LLC, part of Springer Nature 2022 Precision m

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Methods to Detect Nitric Oxide and Reactive Nitrogen Species in Biological Sample,ch as nitric oxide (NO), nitrite, nitroxyl, s-nitrosothiols, and reactive oxygen species (ROS) such as peroxides, superoxide, and hydroxyl radicals. NO being the main player among RNS induced altered cellular molecules and metabolisms, thus making it important to understand and detect the generation

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Study of Rotary Cell Culture System-Induced Microgravity Effects on Cancer Biomarkers,lore the curiosity. There are several factors in space which are different from that of the earth’s atmosphere, including gravity, cosmic radiation, extreme temperatures, and heavy ions. The space environment consists of very less gravitational force, which is often termed as microgravity. Micrograv

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A Calcium Imaging Approach to Measure Functional Sensitivity of Neurons,ffect on the quality of life in cancer patients. However, the mechanisms underlying chemotherapy and radiation therapy associated with pain are not well understood. Pain sensations are mediated through sensory neurons whose cell bodies are located in the dorsal root ganglia (DRG). Pain mediators act

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Method of Preparation of Cigarette Smoke Extract to Assess Lung Cancer-Associated Changes in Airway comprised of several toxic components, is known to cause oxidative stress and inflammation-induced lung damage. Since airway epithelial cells act as the primary barrier, they protect the lung tissues from environmental insults, including CS. Upon exposure to these insults, airway epithelial cells a

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Co-Immunoprecipitation-Blotting: Analysis of Protein-Protein Interactions,s. Commercial availability of Dynabeads. Protein A magnetic beads provides a fast, convenient, and efficient method for protein interaction studies by Co-IP followed by immunoblotting (Co-IP-blotting). Recently, the Co-IP-blotting technique helped us to investigate complicated protein interactions/n

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Methods to Assess Oxidative DNA Base Damage Repair of Apurinic/Apyrimidinic (AP) Sites Using Radioae genome, and this unrepaired DNA base damage leads to various cellular consequences. The oxidative stress-mediated DNA base damage is involved in various human disorders like cancer, cardiovascular, ocular, and neurodegenerative diseases. Base excision repair (BER) pathway, one of the DNA repair pa

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Determining the Size Distribution and Integrity of Extracellular Vesicles by Dynamic Light Scatteriuptake induces changes in the biological functioning and phenotypes of the recipient cell. Thus, there has been a great deal of interest in understanding their roles in the pathobiology of benign diseases and cancer. Moreover, EVs carry the molecular signatures of the donor cells, and therefore, the

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Characterization of Exosomal Surface Proteins by Immunogold Labeling,r cellular metabolic waste removal to maintain cellular homeostasis. Exosomes secreted by cancer cells play an important role in supporting tumor growth and metastasis by communicating with other cells in the tumor microenvironment and distant sites. Several studies have reported that the exosomes s