Titlebook: Calcium Signalling; Methods and Protocol Anna Raffaello,Denis Vecellio Reane Book 2019 Springer Science+Business Media, LLC, part of Spring

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Methods to Measure Intracellular Ca2+ Concentration Using Ca2+-Sensitive Dyes,will be first presented. Then, the implementation of Fura-2 to detect [Ca.]. in two specific cell types, namely, human adrenocortical cells and primary skin fibroblasts, will be discussed in more particulars. Finally, the procedure to monitor Ca. influx through the plasma membrane using Fura-2 will be described.

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In Vivo Light Sheet Fluorescence Microscopy of Calcium Oscillations in ,,le alignment and image acquisition are detailed. Finally, the image processing steps to analyze calcium oscillations are discussed, with particular emphasis on ratiometric calcium imaging in . root hairs.

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Measuring Calcium and ROS by Genetically Encoded Protein Sensors and Fluorescent Dyes,the most prominent ROS (hydrogen peroxide, H.O.) using genetically encoded protein sensors and fluorescent dyes. We also provide guidelines on how to simultaneously detect Ca. and H.O. and how to examine the influence of Ca. signals on cellular ROS production and vice versa.

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Book 2019yses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introduction

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Ex Vivo Measurements of Ca2+ Transients in Intracellular Compartments of Skeletal Muscle Fibers by mitochondrial Ca. transients in the same fiber. Probe encoding plasmids are expressed in flexor digitorum brevis (FDB) muscles by means of the in vivo electroporation technique. Measurements are then performed ex vivo in isolated single myofibers.

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Book 2019bleshooting and avoiding known pitfalls..Comprehensive and cutting-edge, .Calcium Signalling: Methods and Protocols. is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research..

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In Vivo Monitoring of Ca2+ Uptake into Subcellular Compartments of Mouse Skeletal Muscle,roscopy as well as force transducers and associated hardware for data acquisition. Information on how to determine subcellular localization of the genetically encoded Ca. sensors and on how to calibrate the ratiometric data in a semiquantitative manner is given in the final paragraphs.