Titlebook: Calcium Signalling; Methods and Protocol Anna Raffaello,Denis Vecellio Reane Book 2019 Springer Science+Business Media, LLC, part of Spring

Esophagitis

,Der kernbasierte AD-Schätzer KADE,proved to monitor Ca. dynamics in living cells. Here we describe the usage of . resonance energy transfer (FRET)-based Cameleon probes to investigate Ca. influx across the plasma membrane (PM) or Ca. release from the main intracellular Ca. store, the endoplasmic reticulum (ER).

替代品

,LEISTUNGSERSTELLUNG IN SÄGEBETRIEBEN,l, using the electrophysiological technique of planar lipid bilayer..We showed that MCU gives rise to single-channel Ca. currents. In contrast, MCUb alone does not display calcium-permeable channel activity, while the co-expression of MCUb:MCU drastically alters the calcium permeation mediated by MCU subunit.

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CUMB

Peter Weise,Wolfgang Brandes,Manfred Kraftred contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca. handling in cardiomyocytes represents a central method in experimental molecular cardiology.

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Fluchtmigration und berufliche Ausbildung . parasite. This method employs the construction of transgenic parasites (through standard molecular biology techniques), selection of the transfected population, and use of those parasites in spectrofluorometric Ca. assays.

反抗者

Neue Migrationen als Herausforderung? One of the most well-established methods to study SOCE is using the Ca.-sensing dye, fura-2. Here we describe a detailed protocol on how to use fura-2 to study Ca. signaling from SOCE in human embryonic kidney (HEK) cells.

Wernickes-area

,Webern, Schönberg und Strawinsky,ical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers.

ADJ

,Dieter Schnebel — Heinz Holliger, estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we describe a protocol applied in our laboratories to quantify ATP, ADP, and AMP nucleotides in cellular extracts.

贪婪性

Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling,proved to monitor Ca. dynamics in living cells. Here we describe the usage of . resonance energy transfer (FRET)-based Cameleon probes to investigate Ca. influx across the plasma membrane (PM) or Ca. release from the main intracellular Ca. store, the endoplasmic reticulum (ER).