Titlebook: CRISPR-Cas Methods; Volume 2 M. Tofazzal Islam,Kutubuddin Ali Molla Book 2021 The Editor(s) (if applicable) and The Author(s), under exclus

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A Complete Methodology for the Instruction of CRISPR-Based Gene Editing Using a Simplified Cell-Freo system is novel in its ability to present the broad spectrum of gene editing reaction outcomes without the complexity or cost of in vivo cell work. A major variability in inconsistent results arises from the transfection of mammalian cells, often skewing the ratio of successful, precise, and error

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Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants,ure precise processing. This multiplex system is highly flexible, allowing researchers to make modifications based on plant species and project objectives. The use of this multiplexing toolbox will broaden the application of CRISPR-Cas12a in basic and translational research in plants.

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Designing, Performing, and Analyzing CRISPR-Cas9-Mediated Genome Editing Experiments in Leguminous ning of the CRISPR constructs, transformation, screening, and analysis of the data. We have also highlighted different web based tools and resources for assisting genome editing experiments in legumes.

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1949-2448 organism..The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..978-1-0716-1659-8978-1-0716-1657-4Series ISSN 1949-2448 Series E-ISSN 1949-2456

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