Titlebook: CRISPR Gene Editing; Methods and Protocol Yonglun Luo Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 CRISPR-C

导弹

书目名称CRISPR Gene Editing影响因子(影响力)




书目名称CRISPR Gene Editing影响因子(影响力)学科排名




书目名称CRISPR Gene Editing网络公开度




书目名称CRISPR Gene Editing网络公开度学科排名




书目名称CRISPR Gene Editing被引频次




书目名称CRISPR Gene Editing被引频次学科排名




书目名称CRISPR Gene Editing年度引用




书目名称CRISPR Gene Editing年度引用学科排名




书目名称CRISPR Gene Editing读者反馈




书目名称CRISPR Gene Editing读者反馈学科排名

Mortal

死亡率

James Corner,Gilles A. Tiberghientable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. Here we present the protocol for CRISPR/Cas9-mediated integration of a gene expression cassette into a specific genomic locus in CHO cells using homology-directed DNA repair.

马具

Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAsreptococcus pyogenes complexed with chemically modified sgRNAs. We have found this protocol to work very efficiently in numerous cell lines and primary cells that are difficult to transfect by conventional chemical-based transfection methods.

BUST

CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cellstable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. Here we present the protocol for CRISPR/Cas9-mediated integration of a gene expression cassette into a specific genomic locus in CHO cells using homology-directed DNA repair.

软膏

Book 2019 target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included

软膏

CRISPR-gRNA Designapter. The gRNA targets the genome site to be edited, giving great importance to its design to obtain increased efficiency and decreased off-target events. In this chapter, we describe different tools to design suitable gRNAs for a variety of experimental purposes.

借喻

Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery protein and single guide RNA (sgRNA), the key components of the CRISPR genome editing system. Here, we provide a protocol for production and validation of VSV-G-pseudotyped lentiviral vectors for delivery of the CRISPR system and generation of knockout cell lines.

Melodrama

Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9iciency. This chapter covers protocols for the preparation of reagents to target loci of interest, the transfection, and for the genotyping of single cell-derived iPSC clones. Furthermore, we provide a protocol for the convenient generation of plasmids enabling multiplex gene targeting.

Barter

Contemporary Urban Design Thinkinglow transfection efficiency by conventional transfection method. In this chapter, what we present is an easy method by fabricating CRISPR-Cas9 plasmids into nanoparticle system and efficiently delivered into target cells to achieve gene editing.