Titlebook: Baculovirus; Methods and Protocol Manon M.J. Cox Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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https://doi.org/10.1007/978-1-4614-7318-3uction, and centrifugation-based clarification of cell culture lysate. The same protocol can also be applied for rAAV vector production using traditional Three-Bac, Two-Bac, and Mono-Bac platforms without requiring significant changes.

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The Built Environment and Obesityn attractive alternative platform for the production of biologically active monoclonal antibodies. In this chapter, we demonstrate the production of an HIV-1 broadly neutralizing antibody b12 in BEVS. The processes including transfer vector construction, recombinant baculovirus generation, and antibody production and detection are described.

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Childhood Environment and Obesitys including strategies and considerations to successfully optimize and scale-up using BEVS as a tool for production (Fig. 1). As an illustrative case study, we present an example focused on the production of a viral glycoprotein.

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A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Bnd is thus perfectly adapted to parallel expression screening. In this chapter, we detail our standard operating procedures for the preparation of the reagents, the construction and evaluation of baculoviruses, and the optimization of protein production for both intracellularly expressed and secreted proteins.