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Titlebook: Buckwheat; Methods and Protocol Alexander Betekhtin,Artur Pinski Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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期刊全称Buckwheat
期刊简称Methods and Protocol
影响因子2023Alexander Betekhtin,Artur Pinski
视频video
发行地址Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Buckwheat; Methods and Protocol Alexander Betekhtin,Artur Pinski Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive
影响因子This detailed volume describes the most recent methodology for buckwheat research. Buckwheat serves as a promising functional food source containing various phenolic compounds, such as rutin, quercetin, and C-glycosylflavones, which have a positive therapeutic or dietary effect for promoting human health. This book explores techniques for studying this pseudocereal, namely the two most widely cultivated species, .F. esculentum. (common buckwheat) and .F. tataricum. (Tartary buckwheat). Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Practical and comprehensible, .Buckwheat: Methods and Protocols. is an ideal guide for plant scientists working in this field..
Pindex Book 2024
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Callus Induction Followed by Regeneration and Hairy Root Induction in Common Buckwheat,enic hairy roots from buckwheat plants (. Moench.). Callus induction and regeneration are key steps for many biotechnological, genetic, and breeding approaches, such as genetic modification, production of biologically active compounds, and propagation of valuable germplasm. Induction of hairy roots
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Protoplast Isolation, Culture, and Regeneration in Common and Tartary Buckwheat, establishment of research tools based on protoplasts consists of many steps needed for optimization. Here, we describe the culture of morphogenic callus (MC)- and hypocotyl-derived protoplasts of common (. Moench) and Tartary (. (L.) Gaertn.) buckwheat. Protoplasts embedding in agarose matrix and a
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Immunodetection of Cell Wall Components in Studies on Cell Wall Rebuilding in , and ,nd other wall components using specific primary antibodies. This reaction, combined with calcofluor staining, allows to comprehend how the cell wall is rebuilt during the protoplast culture. In this protocol, the method of immunostaining using antibodies against cell wall components based on . and .
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