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Titlebook: Brown Adipose Tissue; Methods and Protocol David A. Guertin,Christian Wolfrum Book 2022 The Editor(s) (if applicable) and The Author(s), un

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期刊全称Brown Adipose Tissue
期刊简称Methods and Protocol
影响因子2023David A. Guertin,Christian Wolfrum
视频video
发行地址Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
学科分类Methods in Molecular Biology
图书封面Titlebook: Brown Adipose Tissue; Methods and Protocol David A. Guertin,Christian Wolfrum Book 2022 The Editor(s) (if applicable) and The Author(s), un
影响因子This detailed volume explores techniques for researching brown adipose tissue (BAT) and the fascinating biology and therapeutic potential of thermogenic adipocytes. The content reflects the advancing technologies in genetics, imaging, and ‘omics strategies that are allowing researchers to probe BAT biology at unprecedented depths and detail, yet it also presents classic physiology principles, which remain the core tenets of BAT biology. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Brown Adipose Tissue: Methods and Protocols. provides perspectives and detailed protocols for the benefit of both new BAT researchers looking for guidance as well as seasoned researchers who would liketo expand their toolkits. .Chapter 12 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..
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https://doi.org/10.1007/978-981-13-7848-5e tissue development and function. Here we report several protocols using the R26R-mTmG reporter mice in thermogenic tissues based on confocal microscopy and fluorescence based flow cytometry. These techniques may be useful to understand the influence of genetic or environmental alterations in brown adipocyte precursors and adipocyte biology.
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