Titlebook: Brain-Derived Neurotrophic Factor (BDNF); Carlos B. Duarte,Enrico Tongiorgi Book 2019 Springer Science+Business Media, LLC, part of Spring

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Methodology for Detecting and Tracking Brain-Derived Neurotrophic Factor Complexes in Neurons Using exes in fixed neurons as well as single-particle tracking (SPT) in live neurons. These methods are valuable for high-resolution localization and quantitation of low-abundance proteins in single neurons and for obtaining nanoscale-resolution, dynamic information of BDNF trafficking for long time durations (min) in live neurons.

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Macmillan Dictionary of Biotechnologyold™ immunostaining method to detect trkB receptors, by using secondary fluorescein-tagged antibodies conjugated with ultrasmall gold particles..Moreover, we describe here how to combine the two procedures in the same tissue samples for BDNF/trkB multiple labelling studies.

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https://doi.org/10.1007/978-1-349-18303-6installation and setup of requisite hardware and software, the use of the software to acquire data, and the analysis of the data. A prerequisite for attempting this protocol is proficiency with 2-photon imaging of live cells and a microscope that is controlled by ScanImage 3.8 (Vidrio Technologies).

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Macmillan Dictionary of Biotechnology 1–8), which are alternatively spliced to the common exon (exon 9) containing the coding sequence (CDS) and the 3′UTR region. Because the 3′UTR sequence of . contains two polyadenylation sites, each transcript has either a short or a long 3′ noncoding tail, generating 22 transcripts in rodents and 3

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Macmillan Dictionary of Biotechnologyhese changes are regulated during aging. We have recently shown that loss of TrkB signaling in striatopallidal enkephalinergic (ENK.) neurons lead to age-dependent spontaneous hyperlocomotion, associated with reduced striatopallidal activation, demonstrating that BDNF-TrkB signaling in striatal ENK.

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https://doi.org/10.1007/978-1-349-18303-6ion in both central CNS and peripheral PNS where it is involved in most neuronal mechanisms, from development to neurodegeneration, cellular growth, neuroprotection, survival, and network signaling. In the last decades, decreased levels of BDNF were directly linked to Parkinson’s, Alzheimer’s, and H

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https://doi.org/10.1007/978-1-349-18303-6s of neurons. This methodology includes the preparation of BDNF-conjugated quantum dot fluorescent probes for sensitive detection of single BDNF complexes in fixed neurons as well as single-particle tracking (SPT) in live neurons. These methods are valuable for high-resolution localization and quant