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Titlebook: Brain Energy Metabolism; Johannes Hirrlinger,Helle S. Waagepetersen Book 2014 Springer Science+Business Media New York 2014 Analytical tec

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发表于 2025-3-21 20:06:22 | 显示全部楼层 |阅读模式
期刊全称Brain Energy Metabolism
影响因子2023Johannes Hirrlinger,Helle S. Waagepetersen
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发行地址Includes techniques to investigate brain energy metabolism in differing levels of cell and tissue complexity.Provides step-by-step detail essential for reproducible results.Contains key implementation
学科分类Neuromethods
图书封面Titlebook: Brain Energy Metabolism;  Johannes Hirrlinger,Helle S. Waagepetersen Book 2014 Springer Science+Business Media New York 2014 Analytical tec
影响因子.Brain Energy Metabolism. addresses its challenging subject by presenting diverse technologies allowing for the investigation of brain energy metabolism on different levels of complexity. Model systems are discussed, starting from the reductionist approach like primary cell cultures which allow assessing of the properties and functions of a single brain cell type with many different types of analysis, however, at the expense of neglecting the interaction between cell types in the brain. On the other end, analysis in animals and humans in vivo is discussed, maintaining the full complexity of the tissue and the organism but making high demands on the methods of analysis. Written for the popular .Neuromethods .series, chapters include the kind of detailed description and key implementation advice that aims to support reproducible results in the lab..Meticulous and authoritative, .Brain Energy Metabolism. provides an ideal guide for researchers interested in brain energy metabolism with the hope of stimulating more research in this exciting and very important field..
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Transport of Lactate: Characterization of the Transporters Involved in Transport at the Plasma Membflux, various techniques such as in vivo measurements of lactate concentrations by magnetic resonance imaging, determination of lactate-induced proton fluxes in brain slices and cell cultures using fluorescent dyes and pH-sensitive microelectrodes, or uptake studies of radioactively labeled lactate
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Primary Cultures of Astrocytes and Neurons as Model Systems to Study the Metabolism and Metabolite scribe methods to generate primary cultures that are highly enriched in astrocytes or cerebellar granule neurons. These cultures are good model systems to investigate the basal metabolism of astrocytes and neurons as well as metabolite export from these cells. As examples for such studies, we descri
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Metabolic Flux Analysis Tools to Investigate Brain Metabolism In Vitro, in vitro and in vivo. These investigations contributed significantly to elucidate neuro-glial metabolic interactions, cerebral metabolic compartmentation, and the individual contribution of neurons and astrocytes to brain energetics. However, many issues in this field remain unclear and/or under de
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Mitochondrial Bioenergetics Assessed by Functional Fluorescence Dyes,lance in the cell, and the ways in which mitochondrial dysfunction impacts on cell physiology represents a key challenge in modern cellular pathophysiology. This requires a study of mitochondrial function in relation to the physiology of the cell, and so has driven approaches to measure and characte
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A Chip Off the Old Block: The Brain Slice as a Model for Metabolic Studies of Brain Compartmentatiotact brain tissue. Metabolic rates are only slightly slower than in heavily anaesthetised brain, and complications introduced by the blood–brain barrier and peripheral metabolism are removed. In this work, we describe how to make and maintain brain tissue slices in biochemical studies, how to use th
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