Titlebook: Bone Marrow and Stem Cell Transplantation; Meral Beksaç Book 2014Latest edition Springer Science+Business Media, New York 2014 HLA.Hematop

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https://doi.org/10.1007/978-3-031-40556-3tential therapeutic benefit following transplantation into the patients. This chapter summarizes some of the essential protocols and assays used at our laboratory for the isolation, culture, differentiation, and characterization of mesenchymal stem cells from the bone marrow and adipose tissue.

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启发

https://doi.org/10.1007/978-3-031-40556-3signal originating from fluorescently (streptavidin–PE) labeled amplicons captured by the beads. Currently, there are two commercially available systems that differ in the scale of probes and the methods used for amplification and denaturation. One of these is described in detail in this chapter.

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Explosive

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https://doi.org/10.1057/9780230615595es for the most common fusion-gene transcripts in acute and chronic myeloid disorders, methods for detections of disease-specific genetic mutated alterations, as NPM1 and FLT3 gene length mutations, and aberrantly expressed genes, as WT1 gene transcripts, are described in detail for daily use.

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macabre

Sequence-Based Typing of HLA: An Improved Group-Specific Full-Length Gene Sequencing Approach,tion using the low-resolution typing result as reference starting point. Group-specific amplification has already been established for DRB. This method enables a cost-efficient, user-friendly SBT approach resulting in a timely unambiguous HLA typing to an ultrahigh resolution level with minimal hands-on time.