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Titlebook: Biotechnology for Fuels and Chemicals; The Twenty-Ninth Sym William S. Adney,James D. McMillan,K. Thomas Klass Conference proceedings 2008

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Effects of Gene Orientation and Use of Multiple Promoters on the Expression of , and , in ,use of a few promoters to obtain high expression. To improve transcriptional control in heterologous expression, we examined how these factors affect gene expression and enzymatic activity in .. We assembled .-xylose reductase (.) and .-xylitol dehydrogenase (.) in four ways. Each pair of genes was
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Bioreactors for H2 Production by Purple Nonsulfur Bacteriaogenase activity found in some photosynthetic bacteria that functions in darkness to shift CO (and H.O) into H. (and CO.). The mass transport of gaseous CO into an aqueous bacterial suspension was the rate-limiting step and the main challenge for bioreactor design. Hollow-fiber and bubbletrain biore
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Solid-state Fermentation of Xylanase from , in a Multi-layer-packed Bed Reactorand the size of inoculum, bed-loading, and aeration is evaluated during the fermentation process. Mycelial inoculum gives more production than conidial inoculum. Increasing the quantity of inoculum enhances slightly xylanase production. Forced aeration induces more sporulation of strain and reduces
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Succinic Acid Production from Cheese Whey using , 130 Zoncentration, pH, and inoculum size on the succinic acid production were studied. The by-product formation during the fermentation process was also analyzed. The highest succinic acid yield of 0.57 was obtained at initial cheese whey concentration of 50 g/L, while the highest succinic acid productiv
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Effects of pH and Temperature on Recombinant Manganese Peroxidase Production and Stability lignin and increases access of the woody structure to synergistic wood-degrading enzymes such as cellulases and xylanases. Recombinant MnP (rMnP) can be produced in the yeast . αMnP1-1 in fed-batch fermentations. The effects of pH and temperature on recombinant manganese peroxidase (rMnP) productio
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Xylanase Production by , D1 Using Maltose as Carbon Source was induced by various carbon sources. It was found that .-maltose is the best inducer of the enzyme synthesis (7.05 U/mg dry biomass at 48 h), while .-glucose and .-arabinose lead to the production of basal levels of xylanase. The crude enzyme solution is free of cellulases, even when the microorg
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