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Titlebook: Biotechnology and Environmental Science; Molecular Approaches S. Mongkolsuk,P. S. Lovett,J. E. Trempy Book 1992 Springer Science+Business M

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Expression of the β-1,3-Glucanase Gene in Yeast Hansenula Polymorpha). When combinedwith athiol reagent, β-1,3-glucanase alone was found to be able to lyse the yeast cell (6). Currently, the most common-used enzyme for digestion of yeast cell walls is an enzyme preparation called Zymolyase, produced from . (5). We have isolated the glucanase gene from this organism and completely determined its sequence (7).
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https://doi.org/10.1007/978-3-531-91015-4eins. Of these the latter has been generally accepted because of its ready application to control a variety of virus diseases in a number of different crops. Because we recently published a review of the current state of the field (Beachy et al, 1990) this brief review will highlight the status of the field.
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https://doi.org/10.1007/978-3-531-91015-4es not have plasmids, the isolation of an ARS site from the genome of C maltosa was attempted so as to construct vectors. Several host-vector systems were constructed to promote reverse genetics in this non-conventional yeast as described in our recent papers shown below.
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