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Titlebook: Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence; Peter H. Bach (Faculty of Science),C. Hugh Reynold Bo

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Application of Confocal Microscopy for the Study of Neuronal Organization in Human Cortical Areas a after intracellular microelectrode injection of Lucifer Yellow (the Lucifer Yellow-method). In some areas pyramid and nonpyramidal neurons were differentiated by the presence of autofluorescent lipofuscin granules. The Lucifer Yellow-method allows the visualization in a brain specimen obtained from
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Comparison of Fluorescent Dye Transfer in Intercellular Communication Studies Using Scrape-Loading ts and energy between cells and participates in cell growth and differentiation. Gap junctions are well-defined structures (Loewenstein, 1979, 1981) and they allow cell-to-cell transfer of molecules both in mammalian cells . and in confluent cells in culture. The gap junction is unique among the epi
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Characterization of the , Embryo Sac Using Fluorescent Probes and Microinjection of Lucifer Yellow in the sporophyte. The two male gametes from the pollen tube fuse with the two female gametes in the embryo sac within the ovule. Structural data from . sp. demonstrated that the plastid rich male gamete preferentially fuses with the egg cell leading to the embryo (Russell, 1985). In maize BMS line,
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Fluorescent Probes for the Assessment of the Subcellular Effects of Chemicals and Disease Processesening spectrum of “vital” fluorescent probes (i.e. fluorophores that do not adversely affect intact cell viablility), many of which are now designed for specific biochemical investigations, has increased our understanding of molecular processes.
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Whole-Cell Patch-Clamping: Introducing Substances into Cells During Electrical Measurements from thmeasuring electrical membrane properties in, for example, the study of hormone signal transduction pathways. An introduction to the patch-clamp technique will be given. The advantages of the patch-clamp technique for electrophysiological studies are reviewed extensively in many different journals; e
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