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Titlebook: Bioremediation; Methods and Protocol Stephen P. Cummings Book 2010 Humana Press 2010 Bioaugmentation.Bioremediation.ecotoxicity.environment

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https://doi.org/10.1007/978-3-662-63271-0nt approaches can be performed are provided, including the specific reagents required (and their preparation), details of the different extraction and acid digestion protocols to be followed and suitable analytical details to allow the measurement of metals by inductively coupled plasma mass spectro
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https://doi.org/10.1007/978-3-319-23347-5esent an MPN- and a real-time PCR method for the quantification of the bacterial gene . (coding for rubredoxin-dependent alkane monooxygenase) in environmental samples. Both approaches enable a rapid culture-independent screening of the . gene in the environment, which can be used to assess the intr
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Anne S. Tsui,Yingying Zhang,Xiao-Ping Chen on 2DE. We identified a set of catabolic proteins, enzymes, and heat shock molecular chaperones associated with the regulatory network that was found to be overexpressed under phenol-stressed conditions. This chapter also offers optimized ideal directions for 2DE, followed by easy-to-follow directi
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Book 2010ort the effective deployment and monitoring of a bioremediation approach. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible proto
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MPN- and Real-Time-Based PCR Methods for the Quantification of Alkane Monooxygenase Homologous Geneesent an MPN- and a real-time PCR method for the quantification of the bacterial gene . (coding for rubredoxin-dependent alkane monooxygenase) in environmental samples. Both approaches enable a rapid culture-independent screening of the . gene in the environment, which can be used to assess the intr
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Two-Dimensional Gel Electrophoresis: Discovering Biomolecules for Environmental Bioremediation, on 2DE. We identified a set of catabolic proteins, enzymes, and heat shock molecular chaperones associated with the regulatory network that was found to be overexpressed under phenol-stressed conditions. This chapter also offers optimized ideal directions for 2DE, followed by easy-to-follow directi
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Application of Bioassays for the Ecotoxicity Assessment of Contaminated Soils,ples, concentration/response relationships may be obtained. Toxicity assays to soil organisms include earthworms, plants, and microorganisms tests. In addition, toxicity assays with soil extracts are recommended. Assays of extracts with algae, daphnia, and fish (in vitro test using fish cell lines)
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