Titlebook: Biophysics of Membrane Proteins; Methods and Protocol Vincent L. G. Postis,Adrian Goldman Book 2020 Springer Science+Business Media, LLC, p

帐单

1064-3745 es to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers..978-1-0716-0726-8978-1-0716-0724-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

极力证明

Book 2020 and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary mate

填料

https://doi.org/10.1007/978-1-349-01728-7ers are central to many important biomedical phenomena, including resistance of cancers and pathogenic microbes to drugs. The protocol described here can be used to quantify protein-ligand and protein-protein interactions for other soluble, membrane-associated and integral membrane proteins.

bypass

Latin America, Media, and Revolutionecule: establishing path independence of this reaction is essential. Here we describe the procedures required to measure and verify path independence for the folding of outer membrane proteins in large unilamellar vesicles.

帽子

Latin America, Media, and Revolutionometry, and interactions with detergents/lipids/drugs. Furthermore, we discuss the sample requirements and experimental considerations unique to integral membrane protein native mass spectrometry research.

妨碍

详细目录

CRACY

Solution X-Ray Scattering for Membrane Proteins, we describe a complete procedure to build a complete model of a transmembrane protein based on a truncated crystallographic model and experimental SEC-SAXS data using refractometry and UV absorption for internal validation of the measurements.

circumvent

Short-Range Distance Measurement by Transition Metal Ion FRET metal such as nickel (Ni.), copper (Cu.), or cobalt (Co.). It can be applied to literally any protein where it is possible to perform site-specific incorporation of a fluorescent molecule. This chapter will explain the use and applications of tmFRET in detail using incorporation of the dye with cysteine chemistry on a purified protein sample.

集合

Quantifying the Interaction of Phosphite with ABC Transporters : MicroScale Thermophoresis and a Novers are central to many important biomedical phenomena, including resistance of cancers and pathogenic microbes to drugs. The protocol described here can be used to quantify protein-ligand and protein-protein interactions for other soluble, membrane-associated and integral membrane proteins.