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Titlebook: Biophysical and Biochemical Aspects of Fluorescence Spectroscopy; T. Gregory Dewey Book 1991 Springer Science+Business Media New York 1991

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期刊全称Biophysical and Biochemical Aspects of Fluorescence Spectroscopy
影响因子2023T. Gregory Dewey
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图书封面Titlebook: Biophysical and Biochemical Aspects of Fluorescence Spectroscopy;  T. Gregory Dewey Book 1991 Springer Science+Business Media New York 1991
影响因子Fluorescence spectroscopy has traditionally found wide application in bio­ chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex­ ploited with great success utilizing both intrinsic and extrinsic probes. Re­ cent applications have built upon these traditional strengths and have re­ sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of fundamental processes. Frequently, fluorescence techniques are tailored to attack a specific biological problem. These new methods in turn produce new physical situations and phenomena which are often of interest to the physical chemist. Thus, progress in one area stimulates renewed interest in other areas. The goal of this book is to provide detailed monographs on the use of fluorescence to investigate problems at the forefront of biochemistry and cell biology. This book is not meant to be a comprehensive survey but rather to highlight areas of recent developments. It is designed to be rea
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https://doi.org/10.1007/978-3-030-20511-9e the fundamental considerations and to provide the reader with a feeling for the practical aspects that must be apprehended before time-resolved methods can be profitably applied to complex biological systems.
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lly occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex­ ploited with great success utilizing both intrinsic and extrinsic probes. Re­ cent applications have built upon these traditional st
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Luminescent Trivalent Lanthanides in Studies of Cation Binding Sites, radius and, as such, are useful as steric probes of systems such as ion channels. Second, their paramagnetic and luminescent properties make them versatile spectroscopic probes of cation binding sites.
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Time-Resolved Fluorescence in Biology and Biochemistry,e the fundamental considerations and to provide the reader with a feeling for the practical aspects that must be apprehended before time-resolved methods can be profitably applied to complex biological systems.
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Fluorescence Quenching Reactions,ormational changes in the biomacromolecule can then be monitored in terms of changes in the accessibility to the quencher of the fluorophore. In addition to such topographical information, in some cases information about the conformational dynamics of globular proteins has been obtained with solute quenching reactions.
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