Titlebook: Biophysical Techniques in Photosynthesis; Volume II Thijs J. Aartsma,Jörg Matysik Book 2008 Springer Science+Business Media B.V. 2008 Prote

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978-90-481-7820-9Springer Science+Business Media B.V. 2008

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The Supramolecular Architecture of the Bacterial Photosynthetic Apparatus Studied by Atomic Force Misubmolecular resolution to be acquired. Recently, AFM has been demonstrated to be the unique tool to image the photosynthetic apparatus in native membranes from different photosynthetic bacteria species. This chapter provides rationales how to image at high resolution a native membrane using the AFM

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Non-Linear Contrast Mechanisms for Optical Microscopyisms. Although being primarily an imaging tool, a microscope can also be used as a spectroscopic instrument that offers means to access spectral properties of individual photosynthetic membranes and pigment-protein complexes with a diffraction limited spatial resolution. When coupled with a femtosec

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Magnetic Resonance Imaging of Plants: Water Balance and Water Transport in Relation to Photosynthetihese techniques are non-destructive and non-invasive and can be used to study the dynamics of plant water relations and water transport, e.g., as a function of environmental (stress) conditions. Non-spatially resolved NMR as well as two-dimensional or even three-dimensional imaging strategies can be

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Crystallization Methods of Membrane Proteins: Practical Aspects of Crystallizing Plant Light-Harvesttures of these complexes are indispensable for a better understanding of the underlying molecular mechanism of light reactions. The key step in the structure determination of these complexes by X-ray crystallography is crystallization. Here we review the major aspects concerning membrane protein cry

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X-ray Crystallography of Photosynthetic Proteinsllization and X-ray structure analysis of the large photosynthetic protein complexes are discussed. In the second part of the chapter, selected structures of the protein complexes are presented and the function of the proteins is briefly discussed based on the structural information.