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Titlebook: Biomembrane Protocols; I. Isolation and An John M. Graham,Joan A. Higgins Book 1993 Humana Press 1993 Biomembran.Peroxisom.Western Blottin

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Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption,s (e.g., size and density). In contrast, immunological techniques rely on biological differences, i.e., the expression of different antigens. The most popular immunological method for subcellular fractionation is immunoadsorption onto a solid phase, which offers the prospect of fast purification in
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Analysis of Fatty Acids by Gas Liquid Chromatography,ngle carboxyl group. FA can be classified as saturated, monounsaturated, or polyunsaturated (.). Saturates have all single carbon-to-carbon bonds. Monounsaturates contain a single . double bond, whereas polyunsaturates have two or more. A list of the commonly occurring natural FA in animal tissues i
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https://doi.org/10.1385/0896032361Biomembran; Peroxisom; Western Blotting; biology; biomembrane; enzymes; glycoprotein; immunology; membrane; m
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https://doi.org/10.1007/978-3-0346-0439-0anes. In many cases, a study of a particular membrane presupposes that it can be isolated by disruption of the cells and fractionation of the resulting homogenate, and then identified unambiguously. The early chapters of this volume, ., are concerned with this fractionation process. Later chapters a
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https://doi.org/10.1007/978-3-658-18838-2are the tubular membranes of the smooth and rough endoplasmic reticulum. Almost without exception, these vesicles retain their normal orientation, i.e., the cytoplasmic face is outermost. This is particularly clear in the case of the rough endoplasmic reticulum, for the ribosomes remain attached on
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